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Day 1: 14 November 2023 (Tuesday), 11.30am to 12.30pmTopic: Spectral flow cytometry
Flow cytometry is essential for rapid cell analysis of protein expressions that are necessary for classification of leukaemia and lymphoma subtypes and rapid detection of residual minimal/measurable disease (MRD) during treatment. Although powerful, most clinical grade conventional flow cytometry machines are limited to 8 to 10 detected proteins per tube, requiring multiple tubes (and more cells) for complete analysis. This limitation is addressed with the next generation of spectral flow cytometry, initially introduced in 2004, and shown to detect over 40 proteins per tube. The University of Alabama at Birmingham incorporates spectral flow cytometry to the clinical test menu with the expected advantages of rapid test panel development/modification, higher MRD sensitivity, reduced sample requirement, and more efficient workflow.
In this lecture, Professor Choi will introduce the basics of spectral flow cytometry and the university’s 2.5 years of experience in its incorporation to its clinical workflow.
Day 2: 16 November 2023 (Thursday), 10.30am to 12.30pmTopic: WHO 2022 classification of myeloid and lymphoid neoplasms
Classification of haematopoietic neoplasms has undergone numerous modifications reflecting increasing understanding. The latest iteration began with 2022 publications detailing 2 classifications (WHO 5th and ICC) and the final book forms anticipated in 2024.
In these lectures, Professor Choi will discuss the paediatric neoplasms in the two 2022 classifications of myeloid and lymphoid neoplasms, emphasising the difference from the previous 2016 WHO 4th revised edition, as well as differences between the two 2022 classifications. These differences may require the use of both classifications in a pathology report and provide a foundation for further study and clarification in the next classification(s).
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