Figure 2: Light microscopy _techniques image 1
Leica TCS SP8 STED 3X
Figure 3: Light microscopy_Leica TCS SP8
The Leica TCS SP8 STED 3X functions both as a laser scanning confocal and a 3D-STED system. Equipped with a tunable white light laser (470-670nm) and a diode (405nm) laser, this system offers high flexibility in dye selection. For STED, 592nm and 660nm depletion lasers allow direct nanoscopy in x,y,z for multi-colour studies of sub-cellular structures. The raw images can be exported to Huygens for further processing.
Nikon N-STORM and Yokogawa CSU-W1 spinning disk
Figure 4: Light microscopy_Nikon N-STORM and Yokogawa CSU-W1 spinning disk
The Nikon N-STORM allows protein –to- protein interaction studies at a molecular level by localisations of individual fluorophores. This is achieved using a high QE iXon EMCCD camera to detect fluorescence. Resolution is enhanced by 10 times in x,y and z compared to a normal optical microscope. Reconstruction of the image can be done using the N-STORM analysis module in NIS elements.
The Yokogawa CSU-W1 spinning disk, together with Zyla 4.2 sCMOS camera, offers fast acquisition as well as a large field of view. Long time lapses of live cell imaging can be performed using a stage top incubator, objective heater and CO2 mixer.
Zeiss Elyra PS.1
Figure 5: Light microscopy_Zeiss Elyra PS.1
The Zeiss Elyra PS.1 is capable of doing both SIM and PALM. By illuminating the sample with gratings and post processing, SIM doubles the image resolution in x,y and z. It is the simplest super resolution technique to implement, with no restrictions on the selection of fluorophores.
PALM allows protein-protein interaction studies at a molecular level by localisations of individual fluorophores. This is achieved using a high QE iXon EMCCD camera to detect fluorescence. Resolution is enhanced by 10 times in x,y and z compared to a normal optical microscope. Reconstruction of the image can be done using the ZEN software.
Leica LMD 7 (laser micro-dissection)
Figure 6: Light microscopy_Leica LMD 7
The Leica LMD 7 isolates specific regions of tissues and cells in both bright field and fluorescence illumination. Collection is by gravity to minimise contamination, thus making the LMD 7 ideal for downstream analysis. Furthermore, the LMD 7 can also do live cell cultures for cloning and re-cultivation.
Zeiss LSM710 Confocal Inverted (A) and Upright (B)
Figure 5: Light microscopy_Zeiss Inverted Figure 6: Light microscopy_Zeiss upright
Both the inverted and upright Zeiss LSM710 laser scanning confocal system are equipped with 405, 458, 488, 514 and 633nm laser lines. Images are acquired using the Zeiss Zen software on both machines. The inverted machine has additional temperature and humidity controllers.