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Flow Cell Sorting Staff-Assisted Sorting

Flow Cell Sorting

Flow cell sorting involves acquiring samples, generating data with the option of retrieving highly purified cell populations post-sort. Sorted cells can then be used for downstream studies requiring sub-culturing, functional assays, DNA/RNA work, single cell-indexed sorting etc.

The lab owns a BD FACS Aria III with a 5-laser set up, capable of resolving 18-colours. The cell sorter is also encased in a Baker Class II Type A2 biosafety cabinet, which serves to better protect samples and our operators from biohazardous aerosols. A team of dedicated and experienced operators will be assigned to take on the flow cell sorting session. Cell sorting services are strictly staff-operated in the facility. Sterile cell sorting is available upon request. Please kindly inform core staff should your experiment require that.

Some of the samples routinely sorted in the core include peripheral blood, bone marrow, cord blood, dissociated human/animal tissue samples, tumors, cardiomyocytes, cell lines, spermatogonia etc.

BD FACS Aria III
• 5-laser set up: 355nm UV, 405nm Violet, 488nm Blue, 561nm Yellow-Green, 640nm Red
• 18-Color Detection: 2-Blue, 3-Red, 6-Violet, 7-UV-Yellow Green
• 4-way sort – only available for 0.2mL (PCR), PCR strips, 1.5mL microfuge and 5mL FACS tubes
• 2-way sort available for 0.2 mL PCR, 1.5 mL microfuge, 5 mL FACS and 15 mL falcon tubes
• 1-way sort (384-well, 96-well, 48-well, 24-well, 12-well, 6-well plates)
• Enclosed in a Class II Type A2 biosafety cabinet

Image 5 Aria.jpg

Image 6 BD FACS Aria III Configuration.jpg