Knowledge Bank

Fluorophore Table
Flurophore	Excitation (nm)	Emission (nm)
AlexaFluor Series
Alexa Fluor 350	442	410
Alexa Fluor 405	401	421
Alexa Fluor 430	434	540
Alexa Fluor 488	499	519
Alexa Fluor 500	503	525
Alexa Fluor 514	517	542
Alexa Fluor 532	530	555
Alexa Fluor 546	561	572
Alexa Fluor 555	553	568
Alexa Fluor 568	579	603
Alexa Fluor 594	591	618
Alexa Fluor 610	610	629
Alexa Fluor 633	632	648
Alexa Fluor 647	652	668
Alexa Fluor 660	663	691
Alexa Fluor 680	680	702
Alexa Fluor 700	696	719
Alexa Fluor 750	752	776
Alexa Fluor 790	782	804
BD Horizon Brilliant™ Blue Reagents
BB515	490	515
BD Horizon Brilliant™ Violet Reagents
BV421	407	421
BV510	405	510
BV605	407	605
BV650	407	650
BV711	407	711
BV786	407	786
BD Horizon Brilliant™ Ultraviolet Reagents
BUV395	348	395
BUV496	348	496
BUV661	348	661
BUV737	348	737
BUV805	348	805
Vio Dyes
VioBlue	405	452
VioGreen	405	520
VioBright 515	488	514
Vio 515	488	514
VioBright FITC	488	522
PE-Vio 615	561	619
PerCP-Vio 700	488	704
PE-Vio 770	561	775
APC-vio770	635	775
DyLight Dyes
DyLight 350	353	432
DyLight 405	400	420
DyLight 488	493	518
DyLight 549	562	576
DyLight 594	593	618
DyLight 633	638	658
DyLight 649	654	673
DyLight 680	692	712
DyLight 750	752	778
DyLight 800	777	794
Fluorescent Proteins*
Y66H	360	442
Y66F	360	508
EBFP	380	440
EBFP2	383	448
Azurite	383	447
GFPuv	385	508
T-Sapphire	399	511
TagBFP	402	457
Cerulean	433	475
mCFP	433	475
ECFP	434	477
CyPet	435	477
Y66W	436	485
dKeima-Red	440	616
mKeima-Red	440	620
TagCFP	458	480
AmCyan1	458	489
mTFP1 (Teal)	462	492
S65A	471	504
Midoriishi-Cyan	472	495
Wild Type GFP	396	475
S65C	479	507
TurboGFP	482	502
TagGFP	482	505
TagGFP2	483	506
AcGFP1	484	510
S65L	484	510
Emerald	487	509
S65T	488	511
EGFP	488	507
Azami-Green	492	505
ZsGreen1	493	505
Dronpa-Green	503	518
TagYFP	508	524
EYFP	514	527
Topaz	514	527
Venus	515	528
mCitrine	516	529
YPet	517	530
TurboYFP	525	538
PhiYFP	525	537
PhiYFP-m	525	537
ZsYellow1	529	539
mBanana	540	553
Kusabira-Orange	548	559
mOrange	548	562
mOrange2	549	565
mKO	548	559
TurboRFP	553	574
tdTomato	554	581
DsRed-Express2	554	591
TagRFP	555	584
DsRed-monomer	557	592
DsRed2-("RFP")	563	582
mStrawberry	574	596
TurboFP602	574	602
AsRed2	576	592
mRFP1	584	607
J-Red	584	610
mCherry	587	610
HcRed1	588	618
mKate2	588	633
Katushka(TurboFP635)	588	635
mKate(TagFP635)	588	635
TurboFP635	588	635
mPlum	590	649
mRaspberry	598	625
mNeptune	600	650
E2-Crimson	611	646
*List is non-exhaustive and should be used as a reference only. Actual specifications vary with manufacturer.
Commonly used Viability Dyes
7AAD	546	647
DAPI	345	455
Propidium Iodide	535	620
Hoechst 33342	343	483
Cellular Function Probes
JC-1	561	529/590
TMRM	561	600
Indo-1	361/330	490/405
DCFH	505	535
Other commonly used Fluorochromes
FITC	495	519
PE	565	575
PE-CY5	565	670
PE-Cy5.5	565	690
PE-CF594	565	612
PE-Dazzle 594	566	610
PE-Texas Red	565	603
PE-Alexa 594	565	618
PE-Alexa 610	565	629
PE-Alexa 700	565	719
PerCP	482	675
PerCP-Cy5.5	482	690
APC	645	660
APC-Cy7	650	774
APC-H7	650	785
APC-Alexa 680	650	702
APC-Alexa 700	650	719
APC-Alexa 750	650	774
APC R-700	652